PhD Student - University College Cork

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PhD Student - University College Cork; Isolation Of Bacterial Genetic Elements Which Interact With Eukaryotic Cells

Alimentary Pharmabiotic Centre

The Alimentary Pharmabiotic Centre is a Science Foundation Ireland (SFI) funded Centre for Science Engineering and Technology (CSET).

APC scientists and clinicians are investigating mechanisms by which intestinal bacteria influence health and disease and are developing new therapies for lifelong debilitating gastrointestinal diseases such as ulcerative colitis, Crohn's disease and irritable bowel syndrome. Positions are currently available in UCC for the following;

PhD studentship:

The human gastro-intestinal tract harbours a highly diverse microbial eco-system which is estimated to contain >1000 bacterial species and >7000 strains.

Functions and metabolic activities of this microbial consortium are implicated in diseases such as colon cancer, ulcerative colitis, and Crohn's disease.

In addition, activities of this microbial ecosystem may be linked to important physiological effects in the host such as regulation of fat storage and oxalate homeostasis.

More recently, irritable bowel syndrome and autistic spectrum disorders have been hypothesized to involve aspects of the gut microbial community.

However the majority of the organisms in the gut are not yet cultured and are thus not available for analysis.

In order to address this issue we have constructed a large (250,000 clones, 40kb insert size) metagenomic library of bacterial DNA isolated from the gut microbiota.

We have been using this library to screen for functions which are of relevance to the gut ecosystem.

This PhD will be concerned with taking this library and further analysing it to identify bacterial genetic elements which show the ability to interact with eukaryotic cells.

The assays will involve high through put screening of the library using robotic system, hence the candidate would ideally have a microbiological background and preferentially some cell culturing experience.

Co-Ordinator For Germ-Free Facility

Technician - Germ-Free Facility

Research Assistant - Robotics/Metagenomics

The closing date for all positions is March.

All positions (including PhD position) are available immediately.

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